CHEMISTRY

Enzymes: Temperature, pH, and Specificity

Hands-on labs, inc. Version 42-0054-00-01

LAB REPORT

PHOTOS – Include THREE digital photos with your lab report, either as separate attachments

to an e-mail or paste into your document.

1. Photo #1 – Ex. #1 – Take a photo of the five glucose strips after dipping,

label them a,b,c,d,e to match the data table #1.

2. Photo #2 – Ex. #2 – Take a photo of the 12 glucose strips after dipping,

label them a,b,c, and the times 5,10,15,20 min. to match the data table #2.

3. Photo #3 – Ex. #3 – Take a photo of the four glucose strips after dipping,

label them a,b,c,d, to match the data table #3.

Exercise 1: Enzyme Specificity

Observations

Data Table 1: Glucose Concentration
Wells Concentration of Glucose
a
b
c
d
e

Experiment

EnzymEs TEmpEraTurE pH spEcificiTy

142

©Hands-On Labs, Inc.

www.LabPaq.com

Questions

A. What determines a person’s ability to digest lactose?

B. Which of the wells showed a positive result for glucose? Explain the results.

C. Explain why testing for glucose is used to determine the activity of the enzyme lactase.

D. Explain the experimental conditions for the five different wells.

Exercise 2: Enzymes and Temperature

Observations

Data Table 2: Presence of glucose in wells indicating lactase activity at various temperatures
Well Time (min) Concentration of Glucose
a 5
10
15
20
b 5
10
15
20
c 5
10
15
20

Questions

A. What happens when an enzyme is boiled? Is this effect reversible?

C. Based on your experiment results, what is the optimal temperature for lactase function?

D. Explain what happens as far as the effectiveness of the enzyme at the freezing temperature. Can this effect be overcome when the temperature rises?

Exercise 3: Enzymes and pH

Observations

Data Table 3: Glucose in wells a-d indicates enzyme activity at various pH levels
Well pH Concentration of Glucose
a 3.5
b 5.0
c 6.8
d 11.0

Questions

A. Graph the data placing glucose concentrations on the y-axis and the pH values on the x-axis.

B. What was the effect of pH on the enzyme lactase? Is this true for all enzymes?

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LAB REPORT

PHOTOS

Include

THREE

digital photos with your lab report, either as separate attachments

to an e

mail or paste into your document.

1.

Photo #1

Ex. #1

Take a photo of the five glucose strips after dipping,

label them a,b,c,d,e to match the data table #1.

2.

Photo #2

Ex. #2

Take a photo of the 12 glucose strips after dipping,

label them a,b,c, and the times 5,10,15,20 min. to match the data table #2.

3.

Photo #

3

Ex. #3

Take a photo of the four glucose strips after dipping,

label them a,b,c,d, to match the data

table #3.

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Experiment

EnzymEs TEmpEraTurE pH spEcificiTy

14

2

©Hands-On Labs, Inc.

www.LabPaq.com

Enzymes: Temperature, pH, and Specificity

Hands-on labs, inc. Version 42-0054-00-01

LAB REPORT

PHOTOS – Include THREE digital photos with your lab report, either as separate attachments

to an e-mail or paste into your document.

1. Photo #1 – Ex. #1 – Take a photo of the five glucose strips after dipping,

label them a,b,c,d,e to match the data table #1.

2. Photo #2 – Ex. #2 – Take a photo of the 12 glucose strips after dipping,

label them a,b,c, and the times 5,10,15,20 min. to match the data table #2.

3. Photo #3 – Ex. #3 – Take a photo of the four glucose strips after dipping,

label them a,b,c,d, to match the data table #3.

Exercise 1: Enzyme Specificity

Observations

Data Table 1: Glucose Concentration

Wells Concentration of Glucose

a

b

c

d

e

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