Biology

Microscopy for Microbiology – Use and Function

Hands-On Labs, Inc. Version 42-0249-00-02

Lab Report Assistant

This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions, diagrams if needed, and data tables that should be addressed in a formal lab report. The intent is to facilitate students’ writing of lab reports by providing this information in an editable file which can be sent to an instructor.

Exercise 1: Getting to Know your Compound Microscope

Data Table 1. Microscope Components.

Letter

Component Name

Component Function

A

B

C

D

E

F

G

H

I

J

K

L

M

Data Table 2. Total Magnification.

Lens

Ocular Magnification

Objective Magnification

Total Magnification

Scanning

Low Power

High Power

Oil Immersion

Data Table 3. Field of View.

Lens

Total Magnification

Field of View (mm)

Field of View (µM)

Scanning

Low Power

High Power

Oil Immersion

Data Table 4. Letter e Viewing Results.

Lens

Photograph

Observations

Scanning

Low

High

Oil Immersion

Questions

A. Describe the details in the slides “Letter e” that become visible as the power changed from scanning power, to low power, to high power.

B. Why is it important to calculate the diameter of the field when first using the microscope?

Exercise 2: Viewing Prepared Microbe Slides

Data Table 5. Prepared Slide Viewing Results.

Slide

Photograph

Total Magnification

Amoeba

Penicillium

Yeast

Spirillium

Bacillus

Coccus

Questions

A. Using the field of view calculated in Exercise 1 for the high power lens, approximately how far across are each of the cells in the Bacteria Coccus Form slide in Data Table 5? Show your calculations.

B. Detail techniques you found helpful for focusing on the various slides in this exercise.

Exercise 3: Preparing Wet-Mount Slides

Data Table 6. Wet-Mount Viewing Results.

Slide

Photograph

Total Magnification

Cheek Cell Smear

Dental Tatar Smear

Questions

A. Describe the similarities and differences between the cheek cell wet mount and dental plaque wet mount.

B. How did the process of preparing wet-mount slides become easier as you prepared the second wet-mount slide of this exercise?

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